What are the necessary conditions to ensure Elisa test results? -Huaqiang Electronic Network

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ELISA is a widely used and efficient detection method, favored by many research institutions for its speed and simplicity. To ensure accurate and reliable results, it's essential to follow the correct procedures. Shanghai Jinma Bio has provided a detailed guide on how to perform ELISA tests properly, covering key steps like sample handling and color development.

First, when handling samples, it's important to place them in the incubator immediately after adding them. If there are multiple samples, they should be processed in batches. Always follow the instructions carefully and strictly control the operation time. Avoid extending the incubation period manually, as this can lead to non-specific binding around the reaction wells, making it difficult to clean thoroughly.

Secondly, the color developer should be prepared just before use. Never use expired developers, and make sure to check the color—TMB developer should appear light blue when ready. After adding the enzyme reagent, gently wipe the surface of the microplate with absorbent paper to avoid contamination.

Shanghai Jinma Bio emphasizes that proper technique is crucial for accurate ELISA results. By maintaining the right amount of detection, you can prevent overloading the reaction plate, which could cause longer washing times and affect the overall outcome.

We appreciate your support and trust in Shanghai Jinma Bio. Thank you for choosing us, and we hope you have a great shopping experience!

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