ELISA is a widely preferred method for testing due to its speed and simplicity, especially among many research institutions. However, correct operation is essential to ensure the accuracy and reliability of the results. Shanghai Jinma Bio has carefully analyzed the entire process, from sample loading to color development, to help users achieve the best outcomes.
The first step in the ELISA procedure is proper sample handling. Once the sample is added, it should be placed in the incubator immediately. If there are multiple samples, they should be processed in batches. It's important to strictly follow the recommended time guidelines to avoid extending the incubation period, which can lead to non-specific binding around the wells, making it difficult to clean thoroughly.
Secondly, when preparing the color developer for ELISA, it’s best to make it just before use. Avoid using expired reagents, as they may not produce accurate results. The TMB color developer should appear light blue to the naked eye, indicating it’s still effective.
After adding the enzyme reagent, it’s advisable to gently wipe the surface of the microplate with absorbent paper. This helps prevent contamination and ensures more accurate readings. Shanghai Jinma Bio emphasizes that proper handling of the detection volume is crucial to avoid overloading the plate, which could result in longer washing times and potential errors.
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